NFYB Antibody

SKU: orb750434

Description

NFYB antibody

Images & Validation

• Tested Applications: ELISA, Gel Supershift Assays, IHC, WB
• Dilution Range: ELISA: 1:5,000 - 1:25,000, Gel Supershift Assays: 0.5 to 1.0 µL, IHC: 1:500, WB: 1:500 - 1:3,000
• Reactivity: Human
• Application Notes:
  This product was tested by immunoblot and found to be reactive against NF-Y (B subunit specific) at a dilution of 1:2,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) . Anti-NF-Y (B subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y. Minimal reactivity was observed against the A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against NF-Y using 0.5 to 1.0 µl per assay. This product is tested for immunohistochemistry in human thymus tissue at 1:500.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Target: NFYB
• Purity: This product was prepared from monospecific antiserum by delipidation and defibrination.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 90 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-NF-Y Antibody, Nuclear transcription factor Y subunit beta, Nuclear transcription factor Y subunit B, CAAT box DNA-binding protein subunit B, NF-YB, HAP3

Quick Database Links

Gene Symbol

NFYB

UniProt

P25208

RefSeq:P25208.2