Mouse IgG F(ab')2 Antibody

SKU: orb346281

Description

Mouse IgG F(ab')2 Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: SDS-PAGE
• Application Notes:
  Mouse IgG F(ab')2 fragment has been tested by SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

Key Properties

• Source: Mouse
• Biological Origin: Mouse
• Isotype: IgG F(ab')2
• Conjugation: Unconjugated
• Purity: Mouse IgG F(ab')2 fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Mouse IgG and anti-Mouse IgG F(ab’)2. No reaction was observed against anti-Mouse IgG F(c) or anti-Pepsin.

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Mouse IgG F(ab')2 fragment is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mouse immunoglobulin Fab; F(ab'); F(ab')2; Fragment antigen-binding