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Description
Research Area
Cell Biology
Images & Validation
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| Tested Applications | IF, WB |
|---|---|
| Dilution Range | WB:1:500-1:2,000, IF:1:50-1:500 |
| Reactivity | Drosophila |
| Application Notes |
Key Properties
−| Host | Gallus |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgY |
| Immunogen | Antigen: Recombinant peptide derived from within residues 220 aa to the N-terminus of Drosophila melanogaster Miranda produced in E. coli. Antigen Sequence: MSFSKAKLKRFNDVDVAICGSPAASNSSAGSAGSATPTASSAAAAPPTVQPERKEQIEKFFKDAVRFASSSKEAKEFAIPKEDKKSKGLRLFRTPSLPQRLRFRPTPSHTDTATGSGSGASTAASTPLHSAATTPVKEAKSASRLKGKEALQYEIRHKNLIESQLSQLDVLRRHVDQLKEAEAKLREEHELATSKTDRLIEALTSENLSHKALNE |
| Target | Miranda |
| Purification | Epitope affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from yolk egg. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CG12249, Dmel\CG12249, mir, Mir, MIR, Mira, MIRA antibody.
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Quick Database Links
Gene Symbol
Miranda
Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence