Ccl4 Antibody

SKU: orb1272750

Description

Ccl4 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, NeA, WB
• Reactivity: Mouse
• Application Notes:
  Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine MIP-1β (100.0 ng/mL), a concentration of 8.0-10.0 μg/mL of this antibody is required.ELISA:Indirect:To detect mMIP-1b by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b.SandwichTo detect mMIP-1b by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine MIP-1b as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMIP-1b. Western Blot:To detect mMIP-1b by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMIP-1b is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMIP-1β. Murine MIP-1β specific antibody was purified by affinity chromatography employing immobilized mMIP-1β matrix.
• Target: Ccl4
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Act-2, Mip1b, Scya4, MIP-1B, AT744.1, C-C motif chemokine 4, Immune activation protein 2, ACT-2

Quick Database Links

Gene Symbol

Ccl4

UniProt

P14097

RefSeq:P14097