HYAL1 Antibody

SKU: orb1538219

Description

HYAL1 Antibody

Images & Validation

• Tested Applications: IHC, IHC-P, WB
• Dilution Range: IHC, IHC-P (5 µg/ml), WB (1 µg/ml)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic peptide located between aa36-85 of human HYAL1 (Q12794, NP_695014). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Mouse, Rat, Elephant, Bat, Horse, Guinea pig (100%); Galago, Marmoset, Rabbit, Pig (92%); Monkey, Dog (85%); Bovine (83%).
• Target: HYAL1
• Purification: Immunoaffinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 0.09% sodium azide, 2% sucrose
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HYAL1, FUS2, Hyaluronidase-1, Hyaluronoglucosaminidase 1, LUCA1, Hyaluronoglucosaminidase-1, Plasma hyaluronidase, LuCa-1, Tumor suppressor LUCA-1, HYAL-1, Lung carcinoma protein 1

Quick Database Links

Gene Symbol

HYAL1