Human ROCK1 Protein, His Tag

SKU: orb3245135

Description

ROCK1 (Rho-associated coiled-coil containing protein kinase 1) is a serine/threonine kinase and a major downstream effector of the small GTPase RhoA. It regulates actin cytoskeleton organization, promoting stress fiber formation, focal adhesion assembly, and contractile force generation. Structurally, ROCK1 contains an N-terminal kinase domain, a central coiled-coil region with a Rho-binding domain, and a C-terminal pleckstrin homology domain. It plays critical roles in cell motility, adhesion, cytokinesis, and apoptosis, and is implicated in cancer progression, cardiovascular diseases, and neurological disorders. ROCK1 activity is tightly controlled by RhoA binding and autoinhibition, making it a potential therapeutic target in multiple pathologies.

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 64.1 kDa. The protein migrates as 60-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: InsectCells
• Target: ROCK1
• Reactivity: Human
• Tag: C-10×His
• Expression Region: Met 1 - Leu 535
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 64.1 kDa
• Purity: 90%

Storage & Handling

• Storage: The product MUST be stored at -70°C or lower upon receipt. -70°C for 3 months under sterile conditions
• Form/Appearance: Liquid
• Buffer/Preservatives: 50 mM Tris, 150 mM NaCl, pH 7.5
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

ROCK1

RefSeq:Q13464