Human HMGB1 protein

SKU: orb391850

Description

Recombinant human HMGB1 protein is produced by our E. coli expression system and the target gene encoding Pro92-Val176 is expressed with a MARI tag at the N-terminus.

Research Area

Autophagic, Cancer, DNA Damage, Epigenetics, Immunology, Infectious Diseases, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: HMGB1
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: MARIDPNAPK RPPSAFFLFC SEYRPKIKGE HPGLSIGDVA KKLGEMWNNT AADDKQPYEK KAAKLKEKYE KDIAAYRAKG KPDAAKKGVV

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μM filtered solution of 50mM HEPES, 500mM NaCl, 0.6mM DTT, pH 7.9.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HMG1; High mobility group protein B1; High mobility group protein 1; HMG-1

Quick Database Links

Gene Symbol

HMGB1

UniProt

P09429

NCBI Gene ID

3146