Human High Affinity IgE Receptor Alpha Chain (FceRIa) Recombinant Protein

SKU: orb3245189

Description

Recombinant fusion protein consisting of two copies of the extracellular part of human high affinity IgE receptor α-chain fused to human serum albumin (HSA) [FcεRIα-HSA-FcεRIα]. The protein is secreted from a recombinant CHO cell line and specifically binds to human immunoglobulin E (IgE) with affinity comparable to the native IgE receptor expressed on mast cells and basophils (KD < 1nM). Among other applications it can be used as functional reagent in allergy research, mast cell / basophil biology and as analytical tool for detection and quantification of biologically active (= receptor binding) IgE in experimental cell supernatants and body fluids.

Images & Validation

• Tested Applications: ELISA, FA, FACS, WB
• Application Notes:
  This product is for research use only. Follow Standard Laboratory Practice when handling this recombinant protein. The product contains 0.1 % sodium azide (NaN3) as a preservative, which has been classified as Xn (harmful) in the European Directive 67/548/EEC and should be handled with care. Flush intensively with water when disposing of this reagent through copper or lead plumbing.

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Key Properties

• Source: Recombinant protein produced in CHO cells
• Purification: Sephadex G-25 Gel Permeation Chromatography
• Purity: > 95%, tested by analytical Gel Permeation Chromatography

Storage & Handling

• Storage: Store undiluted at 4°C. For long term storage, aliquot and store at -20°C or -80°C. Avoid repeated freeze-thawing. The product may be shipped on ice pack or dry ice.
• Buffer/Preservatives: Phosphate buffered saline (PBS); pH = 7.2; 0.1% NaN3 (sodium azide)
• Concentration: 1 mg/ml (Bradford)
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only