Human DEFB4A protein

SKU: orb391616

Description

Recombinant human DEFB4A protein is produced by our E. coli expression system and the target gene encoding Gly24-Pro64 is expressed with a 6His tag at the C-terminus.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: DEFB4A; DEFB4B
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Protein Sequence: GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μM filtered solution of 20mM PB, 130mM NaCl, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DEFB102; DEFB2; DEFB4; Beta-defensin 4A; Beta-defensin 2; BD-2; hBD-2; Defensin, beta 2; Skin-antimicrobial peptide 1; SAP1

Quick Database Links

Gene Symbol

DEFB4A; DEFB4B

UniProt

O15263

NCBI Gene ID

1673, 100289462