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Human CD83 protein

SKU: orb391429

Description

Recombinant human CD83 protein is produced by our Mammalian expression system and the target gene encoding Thr20-Ala143 is expressed with a 6His tag at the C-terminus.

Research Area

Immunology & Inflammation

Images & Validation

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Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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SourceHuman cells
TargetCD83
ReactivityHuman
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Protein SequenceTPEVKVACSE DVDLPCTAPW DPQVPYTVSW VKLLEGGEER METPQEDHLR GQHYHQKGQN GSFDAPNERP YSLKIRNTTS CNSGTYRCTL QDPDGQRNLS GKVILRVTGC PAQRKEETFK KYRAVDHHHH HH

Storage & Handling

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StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized
Buffer/Preservatives0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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CD83 antigen; hCD83; B-cell activation protein; Cell surface protein HB15; CD83

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UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Available Sizes

Select a size below

10 μg
$ 240.00
50 μg
$ 550.00
500 μg
$ 3,550.00
DispatchUsually dispatched within 5-10 working days
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