Human CA3 protein

SKU: orb391336

Description

Recombinant human CA3 protein is produced by our E. coli expression system and the target gene encoding Ala2-Lys260 is expressed with a 6His tag at the C-terminus.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: CA3
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: AKEWGYASHN GPDHWHELFP NAKGENQSPI ELHTKDIRHD PSLQPWSVSY DGGSAKTILN NGKTCRVVFD DTYDRSMLRG GPLPGPYRLR QFHLHWGSSD DHGSEHTVDG VKYAAELHLV HWNPKYNTFK EALKQRDGIA VIGIFLKIGH ENGEFQIFLD ALDKIKTKGK EAPFTKFDPS CLFPACRDYW TYQGSFTTPP CEECIVWLLL KEPMTVSSDQ MAKLRSLLSS AENEPPVPLV SNWRPPQPIN NRVVRASFKL EHHHHHH

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.2 μM filtered solution of 25mM Tris-HCl, 150mM NaCl, pH 8.5.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Carbonic anhydrase 3; Carbonate dehydratase III; Carbonic anhydrase III; CA-III

Quick Database Links

Gene Symbol

CA3

UniProt

P07451

NCBI Gene ID

761