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Description
Research Area
Epigenetics
Images & Validation
−
Item 1 of 1
| Tested Applications | ICC, IF, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:500-1:2000, IHC-P: 1:50-1:200, IF/ICC: 1:100-1:500 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti H4F2 antibody, anti HIST antibody, anti HIST1H4A antibody, anti Histone H4 antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ICC
Immunocytochemistry
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Carlos Silvestre-Roig, Quinte Braster, Kanin Wichapong, Ernest Y. Lee, Jean Marie Teulon, Nihel Berrebeh, Janine Winter, José M. Adrover, Giancarlo Santiago Santos, Alexander Froese, Patricia Lemnitzer, Almudena Ortega-Gómez, Raphael Chevre, Julian Marschner, Ariane Schumski, Carla Winter, Laura Perez-Olivares, Chang Pan, Nicole Paulin, Tom Schoufour, Helene Hartwig, Silvia González-Ramos, Frits Kamp, Remco T. A. Megens, Kerri A. Mowen, Matthias Gunzer, Lars Maegdefessel, Tilman Hackeng, Esther Lutgens, Mat Daemen, Julia von Blume, Hans-Joachim Anders, Viacheslav O. Nikolaev, Jean-Luc Pellequer, Christian Weber, Andrés Hidalgo, Gerry A. F. Nicolaes, Gerard C. L. Wong, Oliver Soehnlein Externalized histone H4 orchestrates chronic inflammation by inducing lytic cell death Nature, 569, 236 (2019)
Applications
In vivo
Reactivity
Mouse
Neutrophil extracellular traps and extracellular histones potentiate IL-17 inflammation in periodontitis J Exp Med, 220, e20221751 (2023)