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HER2 (Phospho-Tyr1221/Tyr1222) Rabbit Polyclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IF, WB |
|---|---|
| Dilution Range | WB: 1:500~1:1000; IF: 1:100~1:200 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Peptide-KLH. Peptide sequence around phosphorylation site of tyrosine1221/1222 (N-L-Y(p)-Y(p)-W) derived from Human HER2 |
| Target | HER2 |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Concentration | 1.0mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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