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TXK Antibody

SKU: orb20187

Description

Goat polyclonal antibody to TXK

Research Area

Signal Transduction

Images & Validation

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Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Porcine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx. 75kDa band in lysates of cell lines Jurkat and MOLT4 after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 61.3kDa according to NP_003319.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

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ClonalityPolyclonal
TargetTXK
Protein SequenceELLRAVTEIAETW
Molecular Weight61.3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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anti TXK antibody, anti TXK tyrosine kinase antibody, anti RLK antibody, anti TKL antibody, anti BTKL antibody, anti PSCTK5 antibody, anti PTK4 antibody, anti PTK4 protein tyrosine kinase 4 antibody, anti tyrosine kinase antibody, anti MGC22473 antibody

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NCBI Gene Details

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No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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