POU4F2 Antibody

SKU: orb79360

Description

Goat polyclonal antibody to POU4F2

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:128000, WB: 0.03 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  WB: Preliminary experiments gave an approx 70kDa band in Rat Retina lysates after 0.03μg/ml antibody staining.Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.2kDa according to NP_599182.1.The 70kDa band was successfully blocked by incubation with the immunizing peptide.We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates.Have any further splice variants/modified forms been reported.Experiment Notes: This antibody is not expected to cross-react to the similar POU4F3 (GeneID 5459)

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Key Properties

• Clonality: Polyclonal
• Target: POU4F2
• Protein Sequence: HGLPSHMGCMSDVD
• Molecular Weight: 43.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti BRN3.2 antibody, anti BRN3B antibody, anti Brn-3b antibody, anti Brn3b POU domain transcription factor antibody, anti POU class 4 homeobox 2 antibody, anti POU domain class 4 transcription factor 2 antibody, anti POU domain protein antibody, anti POU domain, class 4, transcription factor 2 antibody, anti POU4F2 antibody

Quick Database Links

Gene Symbol

POU4F2

NCBI Gene ID

5458