NET1/ARHGEF8 (C Terminus) Antibody

SKU: orb18601

Description

Goat polyclonal antibody to NET1

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:128000, IHC-P: 1-2µg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Tonsil shows staining of fibrous structures. Recommended concentration, 2.5ug/ml.WB: Preliminary experiments gave bands at approx 85kDa in Human Placenta and a very strong band at approx 40-50kDa in Human Skeletal Muscle lysates after 0.1 μg/ml staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the calculated size of 67.7kDa according to NP_001040625.1 and of 61.9kDa according to NP_005854.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported

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Key Properties

• Clonality: Polyclonal
• Target: NET1 / ARHGEF8 (C Terminus)
• Protein Sequence: DKALSGGKRKETLV
• Molecular Weight: 67.7; 61.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NET1 antibody, anti ARHGEF8 antibody, anti NET1A antibody, anti neuroepithelial cell transforming gene 1 antibody, anti Rho guanine nucleotide exchange factor (GEF) 8 antibody, anti guanine nucleotide regulatory protein (oncogene) antibody, anti neuroepithelial cell transforming 1 antibody, anti RP11-116G8.1 antibody, anti Net1 proto-oncogene protein antibody, anti small GTP-binding protein regulator antibody

Quick Database Links

Gene Symbol

NET1 / ARHGEF8 (C Terminus)

NCBI Gene ID

10276