NEBL/nebulette Antibody

SKU: orb20310

Description

Goat polyclonal antibody to NEBL

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA: 1:4000, WB: 1 μg/ml
• Predicted Reactivity: Canine, Human, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 30kDa band in Human Brain (Frontal Cortex) lysates, but not in Human, Mouse and Rat Heart lysates after 1 μg/ml antibody staining. This protein has a calculated MW of 31.2kDa according to NP_998734.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates

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Key Properties

• Clonality: Polyclonal
• Target: NEBL / nebulette
• Protein Sequence: ELQRLKRTQE
• Molecular Weight: 116, 31.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NEBL antibody, anti nebulette antibody, anti FLJ53769 antibody, anti LNEBL antibody, anti MGC119746 antibody, anti MGC119747 antibody, anti bA56H7.1 antibody, anti LIM-nebulette antibody, anti NEBL, actin-binding Z-disc protein antibody, anti OTTHUMP00000019268 antibody, anti actin-binding Z-disk protein antibody

Quick Database Links

Gene Symbol

NEBL / nebulette

NCBI Gene ID

10529