MDFI Antibody

SKU: orb20533

Description

Goat polyclonal antibody to MDFI

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA: 1:4000, WB: 1 μg/ml
• Predicted Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 20kDa band in Human Skeletal Muscle lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25.0kDa according to NP_005577.1. The 20kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

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Key Properties

• Clonality: Polyclonal
• Target: MDFI
• Protein Sequence: YQVSGQRPSGCD
• Molecular Weight: 25
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti I-MF antibody, anti I-mfa antibody, anti inhibitor of MyoD family a antibody, anti MyoD family inhibitor antibody, anti OTTHUMP00000196414 antibody, anti MDFI antibody

Quick Database Links

Gene Symbol

MDFI

NCBI Gene ID

4188