GAPDH (C Terminus) Loading Control Antibody

SKU: orb18774

Description

Goat polyclonal antibody to GAPDH

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.001-0.003 μg/ml, IHC-P: 2.5 μg/ml, IF/ICC: 10μg/ml
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Mouse, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 35kDa band observed in lysates of Human Tonsil, Mouse Spleen, and cell lines HeLa, A431 and 293 (calculated MW of 36.1kDa according to NP_002037). Recommended concentration: 0.01-0.03 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: GAPDH (C Terminus) Loading Control
• Protein Sequence: HQVVSSDFNSDT
• Molecular Weight: 36.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti GAPDH antibody, anti glyceraldehyde-3-phosphate dehydrogenase antibody, anti HGNC:4141 antibody, anti G3PD antibody, anti GAPD antibody, anti MGC88685 antibody, anti OTTHUMP00000174431 antibody, anti OTTHUMP00000174432 antibody, anti aging-associated gene 9 protein antibody, anti glyceraldehyde 3-phosphate dehydrogenase antibody

Quick Database Links

Gene Symbol

GAPDH (C Terminus) Loading Control

NCBI Gene ID

2597