Goat anti-FOXI1 / FKHL10 Antibody

SKU: orb18857

Description

Goat polyclonal antibody to FOXI1

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:2000, WB: 1-3 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: Immunohistochemistry: This product has been successfully used in Mouse Epididymus (Blomqvist et al, EMBO J. 2006 Sep 6;25(17):4131-41. PMID 16932748).WB: Preliminary experiments gave bands at approx 50 kDa and 25 kDa in Human Kidney lysate after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the predicted sizes of 41.0kDa according to NP_036320 (isoform a) and 30.8kDa according to NP_65892 (isofrom b). The bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

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Key Properties

• Clonality: Polyclonal
• Target: FOXI1 / FKHL10
• Protein Sequence: TSGVLYPREGTEV
• Molecular Weight: 41.0; 30.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti FOXI1 antibody, anti forkhead box I1 antibody, anti HFH3 antibody, anti FKHL10 antibody, anti FREAC6 antibody, anti HNF-3/fork-head homolog-3 antibody, anti forkhead-related activator 6 antibody, anti forkhead (Drosophila)-like 10 antibody, anti Forkhead, drosophila, homolog-like 10 antibody, anti hepatocyte nuclear factor 3 forkhead homolog 3 antibody, anti FKH10 antibody, anti MGC34197 antibody, anti forkhead-like 10 antibody

Quick Database Links

Gene Symbol

FOXI1 / FKHL10

NCBI Gene ID

2299