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DKK4 Antibody

SKU: orb18362

Description

Goat polyclonal antibody to DKK4

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:32000, WB: 1-3 μg/ml
ReactivityHuman
Predicted ReactivityCanine, Mouse, Porcine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 30-35kDa band seen in Jurkat lysate. Recommended for use at 1-3 μg/ml. Please note that literature indicates DKK4 is detected as three major immunoreactive species of approx. 40 kDa [form (i)], 30-32 kDa [form (ii)] and 15-17 kDa [form (iii)]. We believe this antibody will recognise 30-32kDa species of DKK4.

Key Properties

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ClonalityPolyclonal
TargetDKK4
Protein SequenceRQHARLRVCQKIEKL
Molecular Weight24.9
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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anti DKK4 antibody, anti dickkopf homolog 4 (Xenopus laevis) antibody, anti DKK-4 antibody, anti hdkk-4 antibody, anti Dickkopf gene 4 antibody, anti dickkopf (Xenopus laevis) homolog 4 antibody, anti MGC129562 antibody, anti MGC129563 antibody, anti dickkopf homolog 4 antibody

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NCBI Gene Details

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No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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