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CTLA + CTLB Antibody

SKU: orb20028

Description

Goat polyclonal antibody to CLTA

Images & Validation

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Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 2.5 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 35kDa and 22-23kDa bands observed in Human Brain (Frontal Cortex) lysates (calculated MW of 23.2kDa according to NP_001825.1). The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol. 2000 Sep;279(3):C833-51.; PMID: 10942733). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

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ClonalityPolyclonal
TargetCTLA + CTLB
Protein SequenceCDFNPKSSKQAKD
Molecular Weight23.7; 27.1; 25.7; 23.2; 25.2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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anti CLTA antibody, anti CTLB antibody, anti clathrin, light chain (Lca) antibody, anti LCA antibody, anti clathrin, light polypeptide (Lca) antibody, anti clathrin, light polypeptide A antibody, anti clathrin, light chain (Lcb) antibody, anti LCB antibody
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

NCBI Gene Details

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No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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