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FZD2/Frizzled 2 Antibody

SKU: orb1538213

Description

FZD2/Frizzled 2 Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Tested ApplicationsELISA, IF, IHC, IHC-P, WB
Dilution RangeIF (10 µg/ml), IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:64000), WB (0.03 - 0.1 µg/ml)
ReactivityBovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rat
Application Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx. 23kD band in Human Colon, Heart and Ovary lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.6kD according to NP_001457.1. The 23kD band was successfully blocked by incubation with the immunizing peptide.

Key Properties

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HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1.
TargetFZD2 / Frizzled 2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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FZD2, Dfrizzled2, Fz2, Frizzled family receptor 2, Frizzled-2, HFz2, Frizzled homolog 2, Fz-2, FzE2, Mfz10, Rfz2, Frizzled 2

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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50 μg
$ 740.00
Free Secondary Antibody (20 ul)0/0

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