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Description
Research Area
Cell Biology
Images & Validation
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| Application Notes |
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, in vivo on expression of CCL20 or IL-6. TLR5 can be stimulated in vitro by Flagellin (NLRC4 mutant) (rec.) at a concentration range of 5-50ng/ml and in vivo at a starting concentration of 0.1-1ug/mouse. Does not activate NLRC4 as tested in bone-marrow mouse macrophages. It can be used as negative control for inflammasome signaling. |
| Target | fliC |
| Tag | His Tag |
| Purity | >95% (SDS-PAGE). Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza). |
| Protein Sequence | The native flagellin [FliC] from Salmonella enterica Serovar Typhimurium strain ATCC14028 (aa 1-495) contains a small deletion at the C-terminus of the protein required for NLRC4 activation and is fused at the N-terminus to a His-tag. |
Storage & Handling
−| Storage | Stable for at least 6 months after receipt when stored at -20°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Contains PBS. Reconstitute with 100 μl sterile water. |
| Concentration | 0.1mg/ml after reconstitution. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−FliC (NLRC4 Mutant)
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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