FAK (phospho-Tyr576/Tyr577) Rabbit Polyclonal Antibody

SKU: orb15015

Description

This is a rabbit polyclonal antibody targeting FAK. It is unconjugated and suitable for use in IF, WB applications. The antibody is reactive with human, mouse, rat samples. Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500~1:1000; IF: 1:100~1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide-KLH. Peptide sequence around phosphorylation site of tyrosine 576/tyrosine 577 (S-T-Y(p)-Y(p)-K-A) derived from Human FAK
• Target: FAK
• Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FADK 1; FAK1; PTK2

Quick Database Links

Gene Symbol

FAK

UniProt

Q05397

Liu, Guangming et al. MELK Accelerates the Progression of Colorectal Cancer via Activating the FAK/Src Pathway Biochem. Genet., (2020)

PubMed: 32472210