F(Ab')2 Biotin Antibody Fluorescein Conjugated

SKU: orb345921

Description

F(Ab')2 antibody (FITC)

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution Range: FC: 1:500-1:2,500, IF: 1:500-1:2,500
• Application Notes:
  F(ab')2 Anti-Biotin Fluorescein Conjugated Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG F(ab')2
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein Biotinylated IgG and Biotinylated Bovine Serum Albumin. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Goat F(ab')2 Anti-Biotin Fluorescein Conjugated Antibody, Goat F(ab')2 Anti-Biotin Antibody Fluorescein Conjugation, Goat Fab2 Anti-Biotin Antibody FITC Conjugation