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Description
Research Area
Signal Transduction
Images & Validation
−| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution Range | IF: 1:50-200; WB: 1:500 - 1:2000.; IHC: 1:100 - 1:300. |
| Reactivity | Canine, Fish, Human, Mouse, Porcine, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Peptide-KLH. Peptide sequence around phosphorylation site of serine 51 (E-L-S(p)-R-R) derived from Human eIF2a |
| Target | eIF2a |
| Purification | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide. |
| Concentration | 1.0mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Eukaryotic translation initiation factor 2 subunit alpha; EIF-2A;
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Quick Database Links
Gene Symbol
eIF2a
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)












![Phospho-eIF2 alpha (Ser51)[Ser52] Antibody](/images/pub/media/catalog/product/e/i/eif2_alpha_phospho-ser51_antibody_orb105986_wb_1.jpg)
