E-Cadherin/Cdh1 Rabbit Polyclonal Antibody

SKU: orb3120263

Description

The E-cadherin/Cdh1 Rabbit Polyclonal Antibody (Carrier-free) is a Carrier-free antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, IF, IHC, and WB applications. This antibody targets the Cadherin-1 in Mouse and Rat samples.

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: Western blot, 0.25-0.5 μg/ml, Mouse Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg /1x10^6 cells, Mouse ELISA, 0.1-0.5 μg/ml
• Reactivity: Mouse, Rat
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: E.coli-derived mouse E-cadherin/Cdh1 recombinant protein (Position: Q23-Q708). Mouse Cdh1 shares 77.9% and 90.7% amino acid (aa) sequence identity with human and rat Cdh1, respectively.
• Target: Cadherin-1
• Molecular Weight: 120-130 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Carrier-free

Storage & Handling

• Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Arc 1; Cadherin 1; CD324; CDH1; CDHE; E cadherin; ECAD; E-cad; E-cadherin; LCAM; UVO; Uvomorulin

Quick Database Links

Gene Symbol

Cadherin-1

UniProt

P09803