DENV-1 Envelope protein E Recombinant Protein (N-His)

SKU: orb3234553

Description

DENV-1 Envelope protein E Recombinant Protein (N-His)

Images & Validation

• Tested Applications: ELISA, SDS-PAGE, WB

Key Properties

• Expression System: E. coli
• Biological Origin: Dengue virus 1 (DENV-1)
• Expression Region: Cys283-Phe672
• MW: 45.0 kDa
• Purity: >90% as determined by SDS-PAGE.

Storage & Handling

• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
• Reconstitution: Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

polyprotein [dengue virus type 1], POLY, added by NCBI staff following more recent annotations of this virus sequence, Flavivirus capsid protein C; pfam01003, Flavivirus polyprotein propeptide; pfam01570, glycoprotein E binding interface [polypeptide binding], Flavivirus envelope glycoprotein M; pfam01004, Flavivirus glycoprotein, central and dimerization domains; pfam00869, Flavivirus glycoprotein, immunoglobulin-like domain; pfam02832, homodimer interface [polypeptide binding], low pH domain interface [polypeptide binding], low pH trimer interface [polypeptide binding], flavivirus envelope glycoprotein E, stem/anchor domain; TIGR04240, Flavivirus non-structural Protein NS1; pfam00948, Flavivirus non-structural protein NS2A; pfam01005, Flavivirus non-structural protein NS2B; pfam01002, Peptidase S7, Flavivirus NS3 serine protease; pfam00949, Flavivirus DEAD domain; pfam07652, C-terminal helicase domain of viral helicase; cd18806, homodimer interface [polypeptide binding], putative ATP binding site [chemical binding], Flavivirus non-structural protein NS4A; pfam01350, added by NCBI staff following more recent annotations of this virus sequence, Flavivirus non-structural protein NS4B; pfam01349, Cap-0 specific (nucleoside-2'-O-)-methyltransferase of flaviviridae; cd20761, nucleic acid substrate binding site [nucleotide binding], SAM binding site [chemical binding], catalytic core domain of RNA-dependent RNA polymerase (RdRp) in the genus Flavivirus, within the family Flaviviridae of positive-sense single-stranded RNA (+ssRNA) viruses; cd23204, inhibitor binding site [chemical binding], conserved polymerase motif F, RNA binding site [nucleotide binding], NTP binding site [chemical binding], conserved polymerase motif A, conserved polymerase motif B, conserved polymerase motif C

Quick Database Links

UniProt

P17763