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DCLRE1C/Artemis Antibody

SKU: orb1538834

Description

DCLRE1C/Artemis Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

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Tested ApplicationsELISA, IHC, IHC-P, WB
Dilution RangeELISA (1:10000 - 1:20000), IHC, IHC-P (1.25 - 2.5 µg/ml), WB (1:500 - 1:2000)
ReactivityHuman
Application Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 1.25-2.5 ug/ml.

Key Properties

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HostGoat
ClonalityPolyclonal
IsotypeIgG
ImmunogenThis affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding an internal region of Human ARTEMIS (DCLRE1C DNA cross-link repair 1C).
TargetDCLRE1C / Artemis
PurificationAffinity purified
ConjugationUnconjugated

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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DCLRE1C, A-SCID, ARTEMIS, DCLREC1C, HSNM1C, SCIDA, RS-SCID, SNM1C, SNM1-like protein, Protein A-SCID, Protein artemis, PSO2 homolog, ASCID, DNA cross-link repair 1C, SNM1 homolog C

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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50 μg
$ 770.00
Free Secondary Antibody (20 ul)0/0

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