Collagen III/COL3A1 Rabbit Polyclonal Antibody

SKU: orb3116826

Description

The Collagen III/COL3A1 Rabbit Polyclonal Antibody (Carrier-free) is a Carrier-free antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, IHC, and WB applications. This antibody targets the Collagen alpha-1 in Human, Mouse, and Rat samples.

Research Area

Cell Biology, Musculoskeletal & Connective Tissue Research, Protein Biochemistry, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml Flow Cytometry(Fixed), 1-3 μg/1x10^6 cells ELISA, 0.1-0.5μg/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Collagen III/COL3A1 recombinant protein (Position: D1222-E1455).
• Target: Collagen alpha-1
• Molecular Weight: 200 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Carrier-free

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

COL3A1; Collagen alpha 1(III) chain; Collagen Type III; EDS4A

Quick Database Links

Gene Symbol

Collagen alpha-1

UniProt

P02461