Colchicine

SKU: orb1310726

Description

Colchicine is a potent natural alkaloid that inhibits microtubule polymerization (IC50 = 3 nM) and acts as a competitive glycine receptor antagonist. It is widely used in research for its anti-inflammatory and immunosuppressive properties, applied in studies of gout, rheumatoid arthritis, Alzheimer's disease models, and NLRP3 inflammasome-related pathologies in both cellular and animal studies.

Research Area

Cell Biology

Key Properties

• CAS Number: 64-86-8
• MW: 399.45
• Purity: >98%
• Formula: C₂₂H₂₅NO₆
• SMILES: COC1=CC2=C(C(OC)=C1OC)C1=CC=C(OC)C(=O)C=C1[C@H](CC2)NC(C)=O
• Target: Cytoskeleton
• Solubility: Soluble in DMSO (up to 25 mg/ml), in Water (up to 25 mg/ml) or in Ethanol (up to 25 mg/ml).

Bioactivity

• Target IC50:
  A10 cells:0.03 μM (EC50)|Microtubule:3 nM|786-O cells:0.022 μM
• In Vivo:
  METHODS: To investigate the antitumor activity, Colchicine (0.1 mg/kg) was orally administered to BALB/c-nu mice bearing the human pharyngeal cancer tumor FaDu every two days for fourteen days. RESULTS: Colchicine was effective in inhibiting tumor growth in a hypopharyngeal cancer model nude mouse without serious complications. METHODS: To investigate the effect of anti-Fas antibody-induced lethality, Colchicine (2 mg/kg) was injected intraperitoneally into C57BL/6 mice, followed by Jo2 antibody (10 μg) 24 h later. RESULTS: All mice treated with Colchicine survived the lethal attack.Colchicine reduced the susceptibility of mice to the lethal effect of Jo2 against Fas antibody.
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• In Vitro:
  METHODS: Human pharyngeal carcinoma cells FaDu and SNU1041 were treated with Colchicine (0.0-1 μM) for 24-72 h. Cell viability was measured by XTT assay. RESULTS: Colchicine treatment was cytotoxic to both FaDu and SNU1041 cell lines in a dose- and time-dependent manner. METHODS: Chorionic villous cells AFCs and amniotic fluid cells CVCs were treated with Colchicine (0.15 μg/mL) for 3-24 h. Apoptosis was detected by Flow Cytometry. RESULTS: Colchicine induced a significant increase in the proportion of annexin V and PI double positive cells.
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• Animal Research:
  a C57BL/6 background are used. To examine the effects of Colchicine on NSAID-induced small intestinal injury, vehicle or Colchicine (1 or 3 mg/kg) is administered orally 30 min prior to indomethacin administration. Mice received intraperitoneal injections of sterilized phosphate buffered saline or mouse recombinant IL-1β (0.1 μg/kg) 3 h after indomethacin treatment. Vehicle or Colchicine (1 or 3 mg/kg) is also administered to NLRP3 / mice before indomethacin administration. The lesion index is evaluated 24 h after indomethacin administration and examined mRNA and protein expression of inflammasome components 6 h after indomethacin administration.
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Storage & Handling

• Storage: RT
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

microtubule, MicrotubuleAssociated, Microtubule/Tubulin, Microtubule Associated, inhibit, Inhibitor, Apoptosis, Autophagy, Colchicine, Colchineos, Colchisol, Colcin

Compound Identifiers

PubChem CID

2833