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14-3-3 gamma/YWHAG Rabbit Polyclonal Antibody

SKU: orb3106334

Description

The 14-3-3 gamma/YWHAG/14 Rabbit Polyclonal Antibody (Carrier-free) is a Carrier-free antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, and WB applications. This antibody targets the 14-3-3 protein gamma in Human, Mouse, and Rat samples.

Research Area

Signal Transduction

Images & Validation

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Tested ApplicationsELISA, FC, WB
Dilution RangeWestern blot, 0.25-0.5μg/ml, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human ELISA, 0.1-0.5μg/ml
ReactivityHuman, Mouse, Rat
Application Notes
For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

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Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenE.coli-derived human 14-3-3 gamma/YWHAG recombinant protein (Position: Y107-A138).
Target14-3-3 protein gamma
Molecular Weight28 kDa
PurificationImmunogen affinity purified.
ConjugationCarrier-free

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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14 3 3 protein gamma; 14 3 3GAMMA; 14-3-3; 14-3-3 GAMMA; KCIP 1; YWHAG

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UniProt Details

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No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 470.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-4 weeks
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