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Chicken IgG (H&L) Secondary Antibody Biotin Conjugated

SKU: orb346919

Description

Chicken IgG (H&L) antibody (Biotin)

Images & Validation

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Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:500,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:20,000
ReactivityGallus
Application Notes
Secondary antibodies are ideal for western blotting, Immunohistochemistry, ELISA, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods

Key Properties

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Antibody TypeSecondary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenAnti-Chicken IgY / IgG whole molecule was produced by repeated immunization with Chicken IgY / IgG whole molecule in rabbit.
PurityBiotin Conjugated Anti-Chicken IgG / IgY secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG / IgY coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum and Chicken IgG / IgY. Some light chain cross reactivity may be observed against chicken immunoglobulins.
ConjugationBiotin

Storage & Handling

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StorageStore secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.5 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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Rabbit anti-Chicken IgG Biotin Conjugated Antibody, Rabbit anti-Chicken IgG Antibody Biotin Conjugation, Chicken Secondary Antibody, Rabbit anti-Chicken IgY Antibody Biotin

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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1.5 mg
$ 420.00
Free Secondary Antibody (20 ul)0/0

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