CDK6 (phospho-Tyr13) Rabbit Polyclonal Antibody

SKU: orb15013

Description

This is a rabbit polyclonal antibody targeting CDK6. It is unconjugated and suitable for use in IF, IHC, WB applications. The antibody is reactive with human, mouse, rat samples. Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide-KLH. Peptide sequence around phosphorylation site of tyrosine 13 (Q-Q-Y(p)-E-C) derived from Human CDK6
• Target: CDK6
• Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Serine/threonine-protein kinase PLSTIRE

Quick Database Links

Gene Symbol

CDK6

UniProt

Q00534

Daniel J Merk 1 2, Foteini Tsiami 1 2, Sophie Hirsch 1 2 3, Bianca Walter 1 2, Lara A Haeusser 1 2 4, Jens D Maile 1 2, Aaron Stahl 5, Mohamed A Jarboui 6 7, Anna Lechado-Terradas 8, Franziska Klose 6 7, Sepideh Babaei 9 2, Jakob Admard 10 11 3, Nicolas Casadei 10 11 3, Cristiana Roggia 10 11 3, Michael Spohn 12 13, Jens Schittenhelm 14 3, Stephan Singer 15 2 3, Ulrich Schüller 12 16 17, Federica Piccioni 18 19, Nicole S Persky 18, Manfred Claassen 9 20 2 21, Marcos Tatagiba 22 4 3, Philipp J Kahle 8 23, David E Root 18, Markus Templin 5, Ghazaleh Tabataba Functional screening reveals genetic dependencies and diverging cell cycle control in atypical teratoid rhabdoid tumors Genome Biol, (2024)

PubMed: 39617889