C-RAF (Phospho-Ser301) Rabbit Polyclonal Antibody

SKU: orb251731

Description

This is a rabbit polyclonal antibody targeting C-RAF. It is unconjugated and suitable for use in WB application. The antibody is reactive with human, mouse, rat samples. Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:500~1:3000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: peptide. Peptide sequence around phosphorylation site of Serine301 (N-L-S(p)-P-T) derived from Human C-RAF
• Target: C-RAF
• Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RAF proto-oncogene serine/threonine-protein kinase EC=2.7.11.1; Proto-oncogene c-RAF; cRaf; RAF; RAF1

Quick Database Links

Gene Symbol

C-RAF

UniProt

P04049