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B2M Antibody (Biotin)

SKU: orb344770

Description

Beta-2-Microglobulin antibody (Biotin)

Research Area

Immunology & Inflammation

Images & Validation

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Tested ApplicationsDOT, ELISA, IHC, WB
Dilution RangeELISA: 1:2,000 - 1:10,000, IHC: 1:500 - 1:3,000, WB: 1:1,000 - 1:5,000
ReactivityHuman
Application Notes
Anti-beta-2-Microglobulin Biotin antibody has been tested by dot blot and is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

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Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenAnti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.
TargetB2M
PurityAnti-beta-2-Microglobulin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b2-Microglobulin [Human Urine]. Cross reactivity against b2-Microglobulin from other sources may occur but has not been specifically determined.
ConjugationBiotin

Storage & Handling

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StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/AppearanceLyophilized
Buffer/PreservativesPreservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration1.0 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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rabbit anti-Beta-2-Microglobulin Antibody biotin Conjugation, biotin conjugated Beta-2-Microglobulin Antibody, B2M antibody, Beta 2 microglobulin precursor antibody, Beta chain of mhc class 1 proteins antibody, Hdcma22p antibody

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UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
DOT
Dot Blot
View Protocol

Available Sizes

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100 μg
$ 860.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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