Bach2 Antibody

SKU: orb345730

Description

Bach2 antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:5000, IP: 1 µg/mL, WB: 1 µg/mL
• Reactivity: Mouse
• Application Notes:
  Affinity purified Bach-2 antibody has been tested for use in ELISA, Immunoprecipitation, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91-140 kDa in size corresponding to Bach2 protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Bach2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminus region of the Bach2 protein.
• Target: Bach2
• Purity: Bach2 antibody is directed against mouse Bach2 (n-terminus) protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest 100% homology with Bach2 protein from mouse. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.97mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Bach2 Antibody, Bach-2, Bach 2, BTB and CNC homology 1, basic leucine zipper transcription factor 2

Quick Database Links

Gene Symbol

Bach2

UniProt

A2RRI0

RefSeq (Protein):NP_001103131.1