AVS Bio - Recombinant Thrombomodulin-(his-TEV)N, human

SKU: T006

Description

Thrombomodulin is an endothelial-specific type I membrane receptor that plays an essential role in maintaining the hemostatic balance. It binds thrombin, forming the thrombomodulinthrombin complex. In the thrombomodulin-thrombin complex, thrombin loses its ability to convert fibrinogen into fibrin. However, it activates protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in the gene of thrombomodulin are a cause of thromboembolic disease, also known as inherited thrombophilia. The mature protein of human thrombomodulin, residues 19 - 516 was over-expressed in HEK293-E cells and purified to homogeneity. The protein has 5 potential N-linked glycosylation sites and 2 potential O-linked glycosylation sites. The protein contains a N-terminal TEV protease cleavable hexahistidine tag. The calculated molecular weight without glycosylation of recombinant human thrombomodulin-(his-TEV)C, residues 19 - 516 including hexahistidine tag, is 54.9 kDa.

Research Area

Hematology, Immunology & Inflammation

Key Properties

• Source: Recombinant
• Purification: IMAC and SEC
• Protein Sequence: marplctllllmatlagalagshhhhhhdydipssenlyfqgsapaepqpggsqcvehdcfalypgpatflnasqicdglrghlmtvrssvaadvislllngdggvgrrrlwiglqlppgcgdpkrlgplrgfqwvtgdnntsysrwarldlngaplcgplcvavsaaeatvpsepiweeqqcevkadgflcefhfpatcrplavepgaaaaavsitygtpfaargadfqalpvgssaavaplglqlmctappgavqghwareapgawdcsvenggcehacnaipgaprcqcpagaalqadgrsctasatqscndlcehfcvpnpdqpgsyscmcetgyrlaadqhrcedvddcilepspcpqrcvntqggfechcypnydlvdgecvepvdpcfranceyqcqplnqtsylcvcaegfapiphephrcqmfcnqtacpadcdpntqascecpegyilddgfictdidecenggfcsgvchnlpgtfecicgpdsalarhigtdcdsgkvdggdsgsgepppsptpgstltppavglvhsg*

Storage & Handling

• Storage: < - 20°C. Thrombomodulin-(his-TEV)N should be stored at - 80°C (stable for at least 1 year). The buffer is PBS without preservative. After thawing it should be stored in appropriate small aliquots at - 20°C or - 80°C (stable for at least 2 months)
• Form/Appearance: solution
• Buffer/Preservatives: PBS, sterile
• Expiration Date: For shelf life and expiration details, please refer to the Certificate of Analysis (CoA).
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

CD141, Fetomodulin

Quick Database Links

UniProt

P07204