AVS Bio - CTLA4-Ecd, Mus Musculus

SKU: C006

Description

CTLA4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. Residues 38-162 of murine CTLA4, containing the extracellular domain - ECD -, were cloned, the protein was over-expressed in HEK293 cells and was purified to homogeneity. The calculated molecular weight of recombinant murine CD40-ECD is 15.1 kDa and it contains an C-terminal hexahistidine tag. Each vial contains 100 µg CD40-ECD at a concentration of 2.5 mg/ml.

Research Area

Cancer Biology

Key Properties

• Protein Sequence: marplctllImatlagalagsiqvtqpsvvlasshgvasfpceyspshntdevrvtvlrqtndqmtevcatftekntvgfldypfcsgtfnesrvnltiqglravdtglylckvelmypppyfvgmgngtqiyvidpepcpdsdfaaahhhhhh*

Storage & Handling

• Storage: Recombinant CTLA4--ECD should be stored at - 80°C (stable for at least 1 year). The protein is stored in PBS. After thawing it should be stored in appropriate small aliquots at - 20°C or - 80°C (stable for at least2 months).
• Form/Appearance: solution
• Expiration Date: For shelf life and expiration details, please refer to the Certificate of Analysis (CoA).
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

cytotoxic T-lymphocyte protein 4 isoform; CTLA4-TM; cytotoxic T-lymphocyte-associated serine esterase-4; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte protein 4; ligand and transmembrane spliced cytotoxic T lymphocyte associated; antigen 4; cytotoxic T-lymphocyte antigen 4; CD152 isoform; cytotoxic T lymphocyte associated antigen 4 short spliced form

Quick Database Links

UniProt

NM_009843; NP_033973; P9793