AVS Bio - Recombinant complement Factor B

SKU: B001

Description

Complement factor B is a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. The mature protein of complement Factor B, residues 26 - 764, was cloned and expressed in HEK293EBNA1 cells and purified to homogeneity (figure 1). It contains an N-terminal hexahistidine tag. Each vial contains 100 µg factor B at a concentration of 1.6 mg/ml.

Key Properties

• Protein Sequence: marplctllImatlagalagshhhhhhgstpwslarpqgscslegveikggsfrllqegqaleyvcpsgfypypvqtrtcrstgswstlktqdqktvrkaecraihcprphdfengeywprspyynvsdeisfhcydgytlrgsanrtcqvngrwsgqtaicdngagycsnpgipigtrkvgsqyrledsvtyhcsrgltlrgsqrtcqeggswsgtepscqdsfmydtpqevaeaflssltetiegvdaedghgpgeqqkrkivldpsgsmniylvldgsdsigasnftgakkclvnliekvasygvkpryglvtyatypkiwvkvseadssnadwvtkqlneinyedhklksgtntkkalqavysmmswpddvppegwnrtrhviilmtdglhnmggdpitvideirdllyigkdrknpredyldvyvfgvgplvnqvninalaskkdneqhvfkvkdmenledvfyqmidesqslslcgmvwehrkgtdyhkqpwqakisvirpskghescmgavvseyfvltaahcftvddkehsikvsvggekrdleiewlfhpnyningkkeagipefydydvaliklknklkygqtirpiclpctegttralrlpptttcqkeellpaqdikalfvseeekkltrkevyikngdkkgscerdaqyapgydkvkdisevvtprflctggvspyadpntcrgdsggplivhkrsrfiqvgviswgwdvcknqkrqkqvpahardfhinlfqvlpwlkeklqdedlgflaaa*

Storage & Handling

• Storage: Factor B should be stored at - 80°C (stable for at least 1 year). The buffer contains 25 mM Tris 150 mM NaCl pH 7.5 without preservative. After thawing it should be stored in appropriate small aliquots at - 20°C or - 80°C (stable for at least 2 months).
• Form/Appearance: solution
• Expiration Date: For shelf life and expiration details, please refer to the Certificate of Analysis (CoA).
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only