ARIH2 Antibody

SKU: orb1247772

Description

ARIH2 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IF, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 60kDa band observed in nuclear lysates of Hela and K562 (Calculated MW of 57.8kDa according to NP_006312.1) Recommended concentration: 1-3µg/mL. Primary incubation was 1 hour. Customer's experiments gave a band at approx 60kDa in lysates in both Human HeLa and Mouse Muscle (IM2 & C2C12) cells. This band disappeared after RNAi knock-down. Calculated MW of 57.8kDa according to NP_006312.1). Recommended concentration: 1-3µg/mL. This antibody has also been successfully used in WB in PMID: 24486325.Immunofluorescence: Customer's experiments showed nuclear staining in DAPI with IF. This antibody has also been successfully used in IF in PMID: 24486325.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-EQRRRTLLKDFHDT
• Target: ARIH2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ARIH2, ariadne homolog 2 (Drosophila), ARI2, TRIAD1, all-trans retinoic acid inducible RING finger, ariadne-2 (D. melanogaster) homolog (all-trans retinoic acid inducible RING finger), FLJ10938, FLJ33921, OTTHUMP00000210388, ariadne homolog 2

Quick Database Links

Gene Symbol

ARIH2

UniProt

O95376

RefSeq (Protein):NP_006312.1