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HPRT1 Rabbit Polyclonal Antibody

SKU: orb1728198
KO/KD
Validated
KO/KD Validated

Description

The HPRT1 Rabbit Polyclonal Antibody is an unconjugated antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, IF, IHC, KO/KD Validated, and WB applications. This antibody targets the Hypoxanthine-guanine phosphoribosyltransferase in Human, Mouse, and Rat samples.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Disease Biomarkers, Hematology, Metabolism Research, Neuroscience, Protein Biochemistry, Stem Cell & Developmental Biology

Images & Validation

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Tested ApplicationsELISA, FC, IF, IHC, KO/KD Validated, WB
Dilution RangeWestern blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml
ReactivityHuman, Mouse, Rat
Application Notes
For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

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Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenE.coli-derived human HPRT1 recombinant protein (Position: Y17-K186).
TargetHypoxanthine-guanine phosphoribosyltransferase
Molecular Weight25 kDa
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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Thrombospondin-1; THBS1; TSP, TSP1

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

UniProt Details

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No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 470.00
Free Secondary Antibody (20 ul)0/0

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