ANLN Antibody

SKU: orb1247798

Description

ANLN Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules. Recommended concentration: 3-10µg/mL.Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10µg/mL. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: WQPDACYKPIGKP
• Target: ANLN
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ANLN, Scraps, ANILLIN, anillin, actin binding protein (scraps homolog, Drosophila), anillin (Drosophila Scraps homolog), actin binding protein, anillin, actin binding protein, DKFZp779A055, scra

Quick Database Links

Gene Symbol

ANLN

UniProt

Q9NQW6

RefSeq (Protein):NP_061155.2, NP_001271231.1, NP_001271230.1