AMPKa1/AMPKa2 Rabbit Polyclonal Antibody

SKU: orb14583

Description

This is a rabbit polyclonal antibody targeting AMPKa1/AMPKa2. It is unconjugated and suitable for use in ELISA, IF, IHC, WB applications. The antibody is reactive with Bovine, Fish, human, monkey, mouse, rat samples. Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:200
• Reactivity: Bovine, Fish, Human, Monkey, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide-KLH. Peptide sequence around aa. 181~185/170~174 (L-R-T-S-C) derived from Human AMPKα1/AMPKα2
• Target: AMPKa1/AMPKa2
• Purification: Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AMPK, AMPKa1

Quick Database Links

Gene Symbol

AMPKa1/AMPKa2

UniProt

Q13131

Li Xu 1, Jie Su 2, Caili Du 3, Hui Liu 4, Wei Lv Ghrelin suppresses hypoxia/reoxygenation-induced H9C2 cell pyroptosis via NLRP3 Cell Mol Biol (Noisy-le-grand) ., (2024)

PubMed: 38158667

Applications

IF

Reactivity

Rat

Cui, Wei et al. Circular RNA DOCK1 downregulates microRNA-124 to induce the growth of human thyroid cancer cell lines Biofactors, (2020)

PubMed: 32584497

Wu, Jingsong et al. Roles of electro-acupuncture in glucose metabolism as assessed by 18F-FDG/PET imaging and AMPKα phosphorylation in rats with ischemic stroke Int J Mol Med, 40, 875-882 (2017)

PubMed: 28713979