Alpha-1-Trypsin Antibody Biotin Conjugated

SKU: orb344285

Description

Alpha-1-Trypsin antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:50,000, WB: 1:2,000 - 1:10,000
• Reactivity: Human
• Application Notes:
  Anti-Alpha-1-Anti-Trypsin has been tested by ELISA and western blot. This product is assayed against 1.0 ug of a-1-anti-Trypsin in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:29,000 of the reconstitution concentration is suggested for this product. 10-20 moles Biotin per mole. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 100 µL

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: a1-Anti-Trypsin [Human Plasma]
• Target: SERPINA1
• Purity: Anti-Alpha-1-Anti-Trypsin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified a-1-anti-Trypsin [Human Plasma]. Cross reactivity against a-1-anti-Trypsin from other sources is unknown.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Alpha-1-Trypsin Antibody biotin Conjugation, biotin Conjugated goat anti-Alpha-1-Trypsin Antibody, A1AT antibody, AAT antibody, Alpha 1 antiproteinase antibody, Alpha 1 antitrypsin antibody, Alpha 1 protease inhibitor antibody

Quick Database Links

Gene Symbol

SERPINA1

UniProt

P01009

RefSeq (Protein):NP_000286.3