Akt (Phospho-Thr308) Rabbit Polyclonal Antibody

SKU: orb5681

Description

This is a rabbit polyclonal antibody targeting Akt. It is unconjugated and suitable for use in IF, IHC, WB applications. The antibody is reactive with human, mouse, rat samples. Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide-KLH. Peptide sequence around phosphorylation site of threonine 308 (M-K-T(p)-F-C) derived from Human Akt
• Target: Akt
• Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C-AKT; PKB; PKB-alpha; RAC; RAC-PK-alpha

Quick Database Links

Gene Symbol

Akt

UniProt

P31749