Beta Actin/ACTB Rabbit Polyclonal Antibody

SKU: orb3081440

Description

The beta Actin/ACTB Rabbit Polyclonal Antibody (Carrier-free) is a Carrier-free antibody raised in rabbit. It is purified from rabbit antiserum by affinity chromatography using phospho peptide and suitable for use in WB applications. This antibody targets the Actin, cytoplasmic 1 in Gallus, Human, Monkey, Mouse, Rat, and Zebrafish samples.

Research Area

Cancer Biology; Musculoskeletal & Connective Tissue Research, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat, Chicken, Zebrafish
• Reactivity: Gallus, Human, Monkey, Mouse, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin, identical to the related rat and mouse sequences.
• Target: Actin, cytoplasmic 1
• Molecular Weight: 42 kDa
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Conjugation: Carrier-free

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ACTB; Beta-actin; B actin; Beta actin; PS1TP5BP1; β actin; BRWS1

Quick Database Links

Gene Symbol

Actin, cytoplasmic 1

UniProt

P60709