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ACK1 (Phospho-Tyr284) Rabbit Polyclonal Antibody

SKU: orb191531

Description

This is a rabbit polyclonal antibody targeting ACK1. It is unconjugated and suitable for use in IF, IHC, WB applications. The antibody is reactive with human, mouse samples. Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsIF, IHC, WB
Dilution RangeWB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:200
ReactivityHuman, Mouse

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
ImmunogenPeptide-KLH. Peptide sequence around phosphorylation site of tyrosine 284 (D-H-Y(p)-V-M) derived from Human ACK1
TargetACK1
PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration1.0mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ACK1; Activated p21cdc42Hs kinase; EC 2.7.10.2; kinase ACK1;

Similar Products

  • Phospho-Ack1 (Tyr284) Rabbit Polyclonal Antibody [orb5664]

    ELISA,  IF,  IHC-Fr,  IHC-P,  WB

    Bovine, Canine, Human, Mouse, Porcine, Rat

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
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UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol

Available Sizes

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50 μl
$ 260.00
100 μl
$ 360.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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