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Featured
Description
Research Area
Amino Acid Metabolism, Oxidative Stress, Vitamins and Minerals
Images & Validation
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Item 1 of 1
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | All |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated 4-Hydroxynonenal |
| Molecular Weight | 66 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Customer Validated Data
− The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution
Latest Experiments
10 ResultsDilution
Sample
Summary
IHC
Rat
1:100
Pancreatic and lung tissues from male Sprague-Dawley rats
Paraffin-embedded tissue sections were deparaffinized, subjected...
WB
Rat
1:1000
Pancreatic and lung tissues from male Sprague-Dawley rats
Sample preparation for western blot involved...
IHC
Mouse
-
Paraffin-embedded (FFPE) lung sections from mouse
Lung tissues were fixed in 4%...
WB
Mouse
1:2000
Primary mouse microglia
Sample preparation involved lysing cells in...
IF
Mouse
-
Mouse intervertebral disc (IVD) tissues
Sample preparation involved deparaffinization and rehydration...
Previous
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−4-Hydroxy-2-Nonenal; 4Hydroxynonenal; 4-Hydroxynonenal; HNE; 4HNE; 4-HNE; (E)-4-Hydroxy-2-nonenal; 2-Nonenal, 4-hydroxy-, (2E)-; (2E)-4-Hydroxy-2-nonenal; 4-Hydroxy-2(E)-nonenal; ; 4-HNE/4-Hydroxynonenal;
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
Filter by Applications
Filter by Species
Jie Chu # 1, Jun Jiang # 1 2, Xin Fan # 1 3, Jun Liu 1, Ke Gao 1 4, Yu Jiang 1, Mengxuan Li 1, Wenjin Xi 5, Lu Zhang 1, Ka Bian 6, Angang Yang 7, Rui Zhang A novel MYC-ZNF706-SLC7A11 regulatory circuit contributes to cancer progression and redox balance in human hepatocellular carcinoma Cell Death Differ, (2024)
Applications
IHC
Reactivity
Human
Fen Zheng 1, Jian-Zhen Lei 2, Jing-Xiao Wang 2, Xiao-Yu Xu 2, Bing Zhou 3, Rui Ge 2, Min Dai 2, Hong-Ke Dong 2, Nan Wu 2, Yue-Hua Li 4, Guo-Qing Zhu 5, Ye-Bo Zhou Crucial roles of asprosin in cisplatin-induced ferroptosis and acute kidney injury Free Radic Biol Med ., (2024)
Vedat Uruc, Ibrahim Gokhan Duman, Serkan Davul, Raif Özden, Ramazan Gonenci, Hasan Gokce, Recep Dokuyucu, Abdurrahman Akdag The Effect of Cape on Steroid Induced Osteonecrosis of the Femoral Head in Rat Model Journal of Hard Tissue Biology, 27, 3 (2018)
Applications
IHC
Reactivity
Rat
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